one-way anova with dunnett’s post hoc multiple comparisons tests (graphpad prism 6 software)  (GraphPad Software Inc)

Journal: Microbiology Spectrum

Article Title: Efficacy of rifapentine and other rifamycins against Coxiella burnetii in vitro

doi: 10.1128/spectrum.01034-24

Figure Lengend Snippet: MICs for selected antibiotics against NMI in THP-1 host cells. NMI C. burnetii was cultured in phorbol myristate acetate-differentiated THP-1 cells in the presence of increasing concentrations of antibiotics to determine the MIC. Data displayed are the mean log 10 genome equivalent per well ± SEM at 7 days post-exposure for each antibiotic at the respective concentration. The MIC was determined as the lowest concentration of antibiotic required to significantly inhibit growth of C. burnetii . Significant inhibition of growth was determined relative to DMSO solvent controls using one-way analysis of variance with Dunnett’s multiple comparisons post hoc test of log 10 transformed data. **** P < 0.0001.

Article Snippet: Significant inhibition of growth was determined relative to DMSO solvent controls using one-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons post hoc test of log 10 transformed data using GraphPad Prism v.9.0.0.

Techniques: Cell Culture, Concentration Assay, Inhibition, Solvent, Transformation Assay

Journal: Microbiology Spectrum

Article Title: Efficacy of rifapentine and other rifamycins against Coxiella burnetii in vitro

doi: 10.1128/spectrum.01034-24

Figure Lengend Snippet: MICs for selected antibiotics against virulent C. burnetii in A-498 host cells. C. burnetii strains ( A ) NMI, ( B ) HPF-GA1, ( C ) CM-SC1, and ( D ) GP-CO1 were cultured in A-498 cells in the presence of increasing concentrations of antibiotics to determine the MIC. Data displayed are the mean log 10 genome equivalent per well ± SEM at 7 (for NMI and HPF-GA1) and 14 (for CM-SC1 and GP-CO1) days post-exposure for each antibiotic at the respective concentration. The MIC was determined as the lowest concentration of antibiotic required to significantly inhibit growth of C. burnetii . Significant inhibition of growth was determined relative to DMSO solvent controls using one-way analysis of variance with Dunnett’s multiple comparisons post hoc test of log 10 transformed data. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Significant inhibition of growth was determined relative to DMSO solvent controls using one-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons post hoc test of log 10 transformed data using GraphPad Prism v.9.0.0.

Techniques: Cell Culture, Concentration Assay, Inhibition, Solvent, Transformation Assay

Journal: Microbiology Spectrum

Article Title: Efficacy of rifapentine and other rifamycins against Coxiella burnetii in vitro

doi: 10.1128/spectrum.01034-24

Figure Lengend Snippet: MBCs for selected antibiotics against virulent C. burnetii in A-498 host cells. C. burnetii strains ( A ) NMI, ( B ) HPF-GA1, ( C ) CM-SC1, and ( D ) GP-CO1 were allowed to recover in A-498 cells for 21 days following a 7-day exposure to antibiotics. GE at day 0 (defined as the day that antibiotics were removed) and at 21 days post-recovery were calculated and transformed by taking the natural log (ln). Data are displayed as the ln difference of the GE per well at day 21 relative to day 0 [ln (GE per well D21) – ln (GE per well D0)]. MBC was determined as the lowest concentration of antibiotic required to render C. burnetii nonviable. C. burnetii was determined to be nonviable if the mean difference in ln transformed GE from day 21 relative to day 0 was significantly different relative to DMSO solvent controls based on one-way analysis of variance with Dunnett’s multiple comparisons post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Significant inhibition of growth was determined relative to DMSO solvent controls using one-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons post hoc test of log 10 transformed data using GraphPad Prism v.9.0.0.

Techniques: Transformation Assay, Concentration Assay, Solvent